JEMI+ Papers

Phosphate deficiency confers transient SDS-EDTA resistance in Escherichia coli K12 ompC knockout mutant

Thursday, September 26, 2019

Ameena Hashimi, Pavneet Kalsi, Prabhreet Sekhon, Celina Sewlochan

Volume 5
Fall 2018 / Winter 2019

OmpC is a general diffusion protein located in the outer membrane (OM) of gram-negative bacteria. It is known to associate with components of the Mla pathway to facilitate OM lipid asymmetry, which protects against harmful external stressors such as antibiotics and detergents.

Deletion of ompF and ompC in Escherichia coli K12 does not affect kanamycin resistance

Thursday, September 26, 2019

Samantha Feng, Tina Liao, Aya Zakaria, Dan Lu Wang

Volume 5
Fall 2018 / Winter 2019

OmpC and OmpF are major Escherichia coli outer membrane general diffusion porins that are involved in the influx of small cationic hydrophilic molecules. It has been suggested that these two porins are involved in resistance to antibiotics such as kanamycin.

Rescue of O16 Antigen expression in E. coli strain MG1655 Prevents Adsorption of T4 Bacteriophage

Thursday, September 26, 2019

Justine Dimou, Jolin Lu, Shannon Pflueger, Eric Toombs

Volume 5
Fall 2018 / Winter 2019

O antigen is the outermost component of lipopolysaccharide and is expressed on the surface of Gram-negative bacteria.

Phosphate deficiency restores SDS-EDTA resistance in an Escherichia coli K12 ompC knockout mutant

Thursday, September 26, 2019

Christiane Boen, Faith Cheung, Milena Kovacevic, Ian Yen

Volume 5
Fall 2018 / Winter 2019

The asymmetric outer membrane (OM) of Gram-negative bacteria protects against external insults, such as antibiotics and detergents. OmpC is a general diffusion porin found in the OM that plays a role in maintaining OM asymmetry. Previous studies have shown that E.

Development of a Rapid, Single-Cell Method of Recombinant Clone Screening Using Flow Cytometry

Thursday, September 26, 2019

Saelin Bjornson, Brian Shim, Grace Kuo, Bruno Freitas

Volume 5
Fall 2018 / Winter 2019

Fluorescence activated cell sorting methods have been adapted to accelerate the isolation of recombinant bacterial clones in the past decade.

Sub-Inhibitory Treatment of Gentamicin in Escherichia coli Decreases T7 Bacteriophage Infectivity and Cell Lysis

Thursday, September 26, 2019

Mahta Amanian, Selina D. Demetrick, Jaime Gabriel G. Gana, Tyler L. Tam

Volume 5
Fall 2018 / Winter 2019

Increasing rates of multidrug resistant Escherichia coli infections have become a growing concern worldwide. With our current repertoire of antibiotics becoming less effective against bacterial infections, novel antimicrobial therapies are needed.

RpoS-Mediated Stress Response Does Not Impact the Rate of T7 Bacteriophage-Induced Lysis of Escherichia coli

Wednesday, September 5, 2018

Kate Huang, Erika Jang, Emily Kamma, Annie Wu​

Volume 4
Fall 2017 / Winter 2018

SUMMARY The alternative sigma factor RpoS is proposed to confer cross-protection against bacteriophage T7-mediated lysis in Escherichia coli after exposure to antibiotics. In E.

Construction of luxCDABE reporter plasmids to investigate regulation of the acrAB and acrEF operons by the AcrS repressor in Escherichia coli BW25113

Wednesday, September 5, 2018

Zoe O’Neill, Olivia Bulka, Megan Marziali, Ryan Nah​

Volume 4
Fall 2017 / Winter 2018

SUMMARY The acrAB and acrEF operons of Escherichia coli encode multidrug efflux pumps capable of efficiently exporting a wide variety of antibiotics. The repression of acrAB and acrEF by regulator, AcrS, has not yet been completely elucidated.

Survival of Caenorhabditis elegans Infected with Escherichia coli DFB1655 is not Affected by a Missense Mutation in dop-1 or Treatment with Chlorpromazine Hydrochloride

Wednesday, September 5, 2018

Christopher G. James, Ofodile Morah, Victoria Panwala, Amin Yarmand​

Volume 4
Fall 2017 / Winter 2018

SUMMARY The O antigen producing Escherichia coli strain DFB1655 L9, has been shown to be pathogenic to Caenorhabditis elegans, with killing rates comparable to the known Caenorhabditis elegans pathogen, Pseudomonas aeruginosa.

The RcsB-dependent Upregulation of rprA Contributes to the Intrinsic Antibiotic Resistance of Escherichia coli Exposed to Antibiotics Targeting Cell Wall Synthesis but not Protein Synthesis

Wednesday, September 5, 2018

Yasaman Jalalkamali, Niknaz Malekafzali, Raisa Shabbir, Tianna Sihota​

Volume 4
Fall 2017 / Winter 2018

SUMMARY The regulation of capsular synthesis (Rcs) phosphorelay system allows Escherichia coli to respond to stress resulting from disruption of the peptidoglycan layer. The cytosolic response regulator, RcsB, regulates the expression of various downstream genes including rprA.

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