JEMI Methods Papers and Videos

RNA extraction of Escherichia coli grown in Lysogeny Broth for use in RT-qPCR

Thursday, July 13, 2017

Mariah Hay, Yu Michael Li, Yvonne Ma​

Volume 1
Fall 2016 / Winter 2017

Herein we describe a method used to effectively extract RNA from the Gram-negative bacterium Escherichia coli grown in Lysogeny Broth (LB). We employ a Proteinase K and enzymatic lysis step to facilitate the release of RNA from bacterial cells.

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