JEMI Methods Papers and Videos

Gram Staining

Wednesday, September 13, 2017

Aaron Naor, Blair Hardman, DJ Hunt​

Volume 1
Fall 2016 / Winter 2017

This video looks at gram staining: a common lab technique used to differentiate bacteria based on their cell walls (Gram Positive vs Gram Negative).
 

Plating

Wednesday, September 13, 2017

Carys Croft, Jordan Chiu, Kevin Ng​

Volume 1
Fall 2016 / Winter 2017

In this video we look at plating methods used in the laboratory.

 

Serial Dilutions

Wednesday, September 13, 2017

Aaron Naor, Blair Hardman, DJ Hunt​

Volume 1
Fall 2016 / Winter 2017

This video looks at the theory and process of preparing serial dilutions and calculating colony counts.

 

Plasmid Preparation

Wednesday, September 13, 2017

Aaron Naor, Blair Hardman, DJ Hunt​

Volume 1
Fall 2016 / Winter 2017

The first part of the video covers the general theory behind the extraction and isolation of plasmid DNA. After, we take a look at how this is done in the laboratory using a plasmid isolation kit.

 

Lysogeny Broth Agar Plates

Wednesday, September 13, 2017

Carys Croft, Jordan Chiu, Kevin Ng​

Volume 1
Fall 2016 / Winter 2017

This video covers the step by step process of preparing Lysogeny Broth (LB) plates.
 

RNA Extraction Tutorial

Wednesday, August 16, 2017

Benson Chang, Arnab Ray, Thomas Tsui

Volume 1
Fall 2016 / Winter 2017

This video has two sections: In the first part, we explore some background theory on RNA preparation and in the second part, demonstrate the steps needed to successfully extract RNA from a sample.

Spot Plating Assay for the Determination of Survival and Plating Efficiency of Escherichia coli in sub-MIC Levels of Antibiotics

Thursday, July 13, 2017

James Wang, Michael Woo, Chris Yan​

Volume 1
Fall 2016 / Winter 2017

Spot plating is an alternative method to spread plating that generates more replicates in shorter periods of time.

Preparation of Calcium Competent Escherichia coli and Heat-Shock Transformation

Thursday, July 13, 2017

Angela Chang, Vivian Chau, Julius Landas, Yvonne Pang​

Volume 1
Fall 2016 / Winter 2017

Calcium chloride heat-shock transformation is a powerful molecular biology technique used to introduce foreign DNA into a host cell. The concept of the technique is to render cells competent using CaCl2 to allow for introduction of plasmid.

Phage Overlay Assay for Quantification of Infectious Bacteriophage Particles

Thursday, July 13, 2017

Francis, Freddy; Miller, Rachel; Nagra, Manveer​

Volume 1
Fall 2016 / Winter 2017

The historical applications of plaque assays included isolation of virus and enumeration of viral particles in a solution.

Antisense RNA Insert Design for Plasmid Construction to Knockdown Target Gene Expression

Thursday, July 13, 2017

Ji, Tom, Lu, Aneka, Wu, Kaylee​

Volume 1
Fall 2016 / Winter 2017

Regulatory RNA molecules are common tools used in bacterial gene regulation. This paper focuses on the steps in designing an antisense RNA component for insertion into a plasmid in order to silence a gene in the plasmid host cell.

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