Current JEMI Papers

Electron Microscopy to visualize T4 bacteriophage interactions with Escherichia coli strain DFB1655 L9, an isogenic derivative of strain MG1655 engineered to express O16 antigen

09/26/2019

Gregory Morgan, David Lim, Paaksum Wong, Blake Tamboline

Volume 24
Fall 2018 / Winter 2019

Research into the interaction between bacteriophage and bacteria has been a subject of interest since their discovery more than a century ago. This field has seen a resurgence in recent years because of its potential implications in the realm of medicine.

Steps toward a luciferase assay system for investigating gene expression

09/26/2019

Adam Mesa, Ru Lan Xu, Annie Yip, Ada Zhang

Volume 24
Fall 2018 / Winter 2019

The luciferase reporter assay is a powerful method of studying gene expression at the transcriptional level because of its high sensitivity and convenience. It detects the activity of luciferase, a light-producing enzyme encoded by the luxCDABE operon.

Escherichia coli K12 Strain JW5917-1, an rcsC Knock Out Strain in the Keio Collection, Displays an Impaired Growth Phenotype Compared to its Isogenic Parent Strain BW25113

09/26/2019

Kyler Kanegawa, Emma McIver, Jackson Moore, Matthew A. Tester

Volume 24
Fall 2018 / Winter 2019

The Regulator of Capsule Synthesis (Rcs) phosphorelay pathway regulates the expression of genes necessary for biofilm formation and stress response within

Slower Growth and Increased Biofilm Formation of Escherichia coli K-12 Stringent Response Mutant, ΔrelA/spoT, under Isoleucine Starvation

09/26/2019

He Huang, Vivian Jiang, Yu Qing Zhou

Volume 24
Fall 2018 / Winter 2019

Biofilm formation contributes to bacterial virulence and its formation is known to be affected by environmental condition and nutrient availability. Cells enter

Supernatants of Escherichia coli K-12 Wildtype Strain and ΔOmpC Mutant Strains do not Confer Resistance to T4 Bacteriophage Infection of K-12 WT cells

09/26/2019

Harvir Grewal, Zhong (Zack) Dang, Yu Sen (Sam) Zhai, JaeHyung (Duke) Sheen

Volume 24
Fall 2018 / Winter 2019

T4 bacteriophage has been well established in its ability to infect Escherichia coli K-12 strains, particularly strain BW25113, through an OmpC-dependent pathway with outer membrane porin C (OmpC) and lipopolysaccharide (LPS) as receptors. Previous studies have suggested that E.

Escherichia coli Nissle 1917 forms biofilm and outgrows Escherichia coli K12 in a temperature-dependent manner

09/26/2019

Jaspreet Gill, Karen Lau, Tina Lee, Hasel Lucas

Volume 24
Fall 2018 / Winter 2019

Planktonic Escherichia coli may grow as biofilms adhered to a surface when environmental stressors are detected. Previous studies utilizing co-culture (culturing of two or more cell populations in contact with one another) and dual-species biofilm-based methods have found that probiotic E.

Developed biofilm assay suggests Escherichia coli Nissle 1917 may mediate biofilm inhibition in Escherichia coli K-12 in liquid co-culture

09/26/2019

Alex Fung, Anderson Li, Helen Lin, Vivian Li

Volume 24
Fall 2018 / Winter 2019

Escherichia coli K12 (K12) is a bacterial species known to form biofilm, which is a community of bacteria residing in a matrix of secreted polysaccharides and

Developing the Antisense Silencing Model for the Investigation of the Mechanism of Resistance of Escherichia coli DFB1655 L9 to T4 Bacteriophage

09/26/2019

Derek Chow, Amanda Clark, Yuan Hung (Oliver) Huang, Si Zhe (Celeste) Ng

Volume 24
Fall 2018 / Winter 2019

O antigen consists of multiple repeating polysaccharide units found on lipopolysaccharide in some Gram-negative bacteria, including Escherichia coli. It is

Lactobacillus rhamnosus GG does not increase Caenorhabditis elegans longevity

09/26/2019

Maye Cheng, Rebecca Dos Remedios, Lucy Lu, Chris Tang

Volume 24
Fall 2018 / Winter 2019

Caenorhabditis elegans is used as a model organism to study longevity. In recent years, C. elegans have helped advance knowledge regarding the human microbiome and host-pathogen interactions due to its characteristic as a bacterivore.

rcsB Does Not Have a Temperature-Dependent Protective Role for Escherichia coli K-12 Against T7 Bacteriophage Lysis

09/26/2019

Sunny Chen, Sophie Guo, Tiffany Leung, Gurkiran Parmar

Volume 24
Fall 2018 / Winter 2019

The Regulator of Capsule Synthesis (Rcs) phosphorelay system in Escherichia coli is a complex thermally regulated two component signal transduction system involved in many cellular functions including capsule production.

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