RpoD is a primary sigma factor in Escherichia coli responsible for expression of lacZ. RpoS is an alternative sigma factor that is upregulated under environmental stress.
Current JEMI Papers
Heat-shock Increases RpoD Dependent β-galactosidase Activity in the Escherichia coli Strains BD792 and B23
Sub-minimum Inhibitory Concentration of Streptomycin and Cephaloridine- Induced Capsular Polysaccharide Production in Escherichia coli K-12 Increases Biofilm Formation in a Wzy-transporter Dependent Manner
Biofilms are bacterial communities that adhere and endure on solid surfaces. They are known to contribute to antibiotic resistance and disease. In our study, we focused on the relationship between capsule and biofilm formation.
Construction of pCXZ14W, a Novel pUC19-derived Plasmid Encoding the rop Gene
When the ColE1-derived cloning vectors pUC19 and pBR322 are co-transformed into Escherichia coli DH5α, pBR322 is excluded from the cells, meaning that overtime the plasmid copy number of pUC19 is higher than that of pBR322.
Antisense RNA Targeting the First Periplasmic Domain of YidC in Escherichia coli Appears to Induce Filamentation but Does Not Affect Cell Viability
YidC is an essential membrane protein in Escherichia coli. It plays a crucial role in the proper insertion and localization of many membrane proteins.
Antisense RNA Targeting the First Periplasmic Domain of YidC Appears to Reduce Cell Growth at 30 Degrees Celcius but Does Not Induce a Filamentous Phenotype
YidC is an essential protein in Escherichia coli that is evolutionarily conserved. It is involved in the proper folding and insertion of many membrane proteins.
Construction of an enlarged pUC19 vector with a rop gene designed to study plasmid maintenance in Escherichia coli
Experimental evidence has demonstrated that when pUC19 and pBR322 are co-transformed into DH5α Escherichia coli, only pUC19 is maintained after a few generations.
Deletion of the Escherichia coli K30 Group I Capsule Biosynthesis Genes wza, wzb and wzc Confers Capsule-Independent Resistance to Macrolide Antibiotics
The Escherichia coli capsule functions to protect bacterial cells from desiccation and environmental stresses. The E. coli group I capsule is polymerized and transported to the surface of the cells through the action of the wza, wzb and wzc gene products.
Characterization of Action and Efficiency of Tn5 Transposase Through Comparative qPCR
We describe initial functional profiling of an in-house cloned Tn5 transposase in the transposition based sequencing library preparation reaction termed tagmentation. We provide the basis for a quantitative procedure to compare efficiency of different Tn5 enzymes in the tagmentation reaction.
Investigating Flow Cytometry as a Potential Method for Real-time Analysis of Gene Expression following Escherichia coli Transformation
Bacterial transformation is commonly detected by growing colonies on selective media. This process is time consuming and labour intensive.