Current JEMI Papers

Effect of Temperature, Inducer Concentration, and Escherichia coli Cytosolic Redox State on MBP-PI2 Expression

07/13/2017

Ian Ang, Antonio Atte, Ben Halim, Jason Jassal​

Volume 21
Fall 2016 / Winter 2017

Type II proteinase inhibitors (PI2) found in potato (Solanum tuberosum) have been observed to confer protective enzymatic activity towards foreign proteases and were shown to have potential in applied contexts including anticancer and hunger suppression.

Copy Number of ColE1-type Plasmids Decreases When rop is Supplied in trans on a Separate Plasmid

07/13/2017

Jose Emmanuel Gozon Gana, Zuroon Hou-Hang Ho, Shraavan Raveendran, Nai Chun Shao​

Volume 21
Fall 2016 / Winter 2017

Previous studies have shown that in Escherichia coli co-transformed with two different plasmids, the expression of the Rop protein from one plasmid can act in trans to decrease the copy number of the other plasmid, an effect referred to as plasmid exclusion.

The Major Periplasmic Domain of YidC May Be Required for Polar Localization of a Green Fluorescence Protein Tagged YidC Variant Protein in Escherichia coli

09/05/2016

Peter Xu, Kevin He, Steven Yan​

Volume 20
Fall 2015 / Winter 2016

Membrane proteins play an essential role in the survival of prokaryotic cells. YidC is a transmembrane protein that functions to insert proteins into the cell membrane either through the Sec translocase dependent pathway or an alternative independent pathway.

Plasmid Mediated Complementation of YidC Using a YidC Variant Fused to Green Fluorescence Protein Supports Growth of Escherichia coli BL21 at 30 Degrees Celcius but not at 37 Degrees Celcius (Note: Erratum associated with this manuscript.)

09/05/2016

Brian Choi, Jeff Jiang, Junsu Kwon, Jimmy Wei

Volume 20
Fall 2015 / Winter 2016

ERRATUM: Follow up studies of the strain described in this study have shown that they are Rhizobium and not E. coli (Hooshmand et al, JEMI, Volume 21, pages 101 – 106).

Synthesis, Cloning, and Sequencing of a Codon Optimized Variant of Proteinase Inhibitor II Designed for Expression in Escherichia Coli

09/05/2016

Emily Fogarty, Arshia Alimohammadi, Jacqueline Siu, Alan Stachowiak​

Volume 20
Fall 2015 / Winter 2016

Proteinase Inhibitor II (PI2) is a potato tuber peptidase with many potential applications including pest control, radiation protection, and hunger suppression. Recombinant production using Escherichia coli represents a cost-effective approach to produce PI2.

Transcriptional Regulation of ompC by Calcium Chloride May Involve envZ

09/01/2016

Sherry (Hao Ting) Bian, Ella (Ke Xin) Liu, Joyce (Tzu Chia) Wang, Yvonne (Seen Yan) Yiu​

Volume 20
Fall 2015 / Winter 2016

The standard method of preparing chemically competent E. coli involves two steps: (1) resuspension and incubation of cells in ice-cold calcium chloride and (2) short heat-shock at 42oC. Exactly how DNA crosses the cell wall and enters the cytoplasm during this treatment is not understood.

Subcloning and Sequencing of the Autotransporter Antigen 43 from Escherichia coli K-12 as an Initial Step Toward Understanding the Relationship Between Ag43 and Capsule in Biofilm Formation

09/01/2016

Ryan Tse, Cybele Go, Stina Lindh​

Volume 20
Fall 2015 / Winter 2016

The autotransporter Antigen 43 (Ag43) is present in numerous Escherichia coli strains and functions in autoaggregation and adhesion leading to biofilm formation. However, biofilm formation and regulation is not well understood.

Escherichia coli K12 Group 2 capsular biosynthesis does not appear to be thermoregulated by an RpoS-dependent mechanism

09/01/2016

Kira Elsbernd, Jonathan Frew, Gunjan Mhapankar, Megan Wheatley​

Volume 20
Fall 2015 / Winter 2016

Capsule production in Escherichia coli strains K12 and K30 requires the transcription factor RfaH which is regulated by unknown mechanisms in E. coli. However, in Salmonella enterica serovar typhi, RfaH is regulated by the stress-response sigma factor RpoS. In E.

Initial Stages of Construction of a Plasmid to Study the Kinetics of Gene Expression at a Single Cell Level Following Uptake of DNA into Escherichia coli

09/01/2016

Ramy A. Slama and Adam S. Ziada​

Volume 20
Fall 2015 / Winter 2016

Bacterial transformation is a technical hallmark of molecular biology, and modifications that could expedite the process could translate into improvements in efficiency.

Development of a Method for Characterization of Bacterial Host DNA Degradation During Nucleolytic Phage Infection – Investigation of Preferential Degradation of Host genomic DNA Using T7 Bacteriophage Infection of Escherichia coli K-12

09/01/2016

Edrick Lin, Olivia Sawatsky, Amanda Wong, Fangwen Zhao​

Volume 20
Fall 2015 / Winter 2016

The T7 bacteriophage lytic life cycle has been thoroughly characterized, however, the possibility of site-specific cleavage of host genomic DNA by T7 nucleases has not been well-investigated and could provide a novel method of probing chromatin structures in bacterial genomic DNA.

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