The group 1 capsule is an extracellular polysaccharide layer that encapsulates Escherichia coli K30 strain E69. Capsule synthesis and transport is dependent on the Wzy biosynthesis system, which is up-regulated when E.
Current UJEMI Papers
Capsular Polysaccharide Production in Escherichia coli K-30 Strain E69 May Increase Following Pretreatment with Tetracycline but not Polymyxin B
09/01/2016
Volume 20
Fall 2015 / Winter 2016
BaeR Is Required for Upregulation of Expression of acrD in Escherichia coli Following Treatment with Subinhibitory Concentrations of Kanamycin
09/01/2016
Volume 20
Fall 2015 / Winter 2016
The AcrAD-TolC efflux pump of Escherichia coli K-12 expels aminoglycosides such as kanamycin from the cell. The expression of genes encoding for proteins which make up the efflux pump are known to be regulated by the two-component systems BaeSR. In E.
Applying the λ-Red Recombinase System to Generate an rcsF Deletion in Escherichia coli DH300
09/01/2016
Volume 20
Fall 2015 / Winter 2016
The outermembrane sensor protein, RcsF, is a component of the Rcs phosphorelay pathway of Escherichia coli, which regulates capsule and biofilm formation. RcsF is shown to be necessary to sense peptidoglycan disruption in the event of exposure to subinhibitory concentrations of antibiotics.
Stringent Response Triggered by Valine-Induced Amino Acid Starvation Does Not Increase Antibiotic Tolerance in Escherichia coli Cultures Grown at Low Cell Density
09/01/2016
Volume 20
Fall 2015 / Winter 2016
The stringent response is a survival mechanism in bacteria that is activated by various environmental stressors, including nutrient limitation such as amino acid starvation.
Investigation of Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) for Detection of Clostridium difficile Toxin A and B From Stools
09/01/2016
Volume 20
Fall 2015 / Winter 2016
Objective: Detection of Clostridium difficile toxins A and B from stools using matrix assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI).
Development of a System to Monitor ompC Transcription in Escherichia coli Using a Green Fluorescence Protein Reporter System
09/01/2016
Volume 20
Fall 2015 / Winter 2016
Transient resistance to antibiotics is believed to be achieved through regulation of outer membrane proteins like OmpC. OmpC is upregulated in response to sublethal doses of the aminoglycoside kanamycin.
Single Deletion of Escherichia coli K30 Group I Capsule Biosynthesis System Component, wzb, Is Not Sufficient to Confer Capsule-Independent Resistance to Erythromycin
09/01/2016
Volume 20
Fall 2015 / Winter 2016
Escherichia coli overcomes different environmental stresses to survive, one of which is cellular death by antibiotics.
AcrS Is a Potential Repressor of acrA Expression in Escherichia coli and Its Deletion Confers Increased Kanamycin Resistance in E. coli BW25113
09/01/2016
Volume 20
Fall 2015 / Winter 2016
AcrAB-TolC is a major efflux pump complex in the resistance nodulation-division family that is responsible for antibiotic resistance in Escherichia coli. Previous research has shown that deletion of the pump component protein, AcrA, leads to decreased drug resistance in E. coli.
Crl and RpoS May Not Be Involved in Cross Protection against Tetracycline in Escherichia coli in Response to Heat Stress
09/01/2016
Volume 20
Fall 2015 / Winter 2016
RpoS is the general regulator of stress response in Escherichia coli and is up-regulated under environmental stress conditions such as heat stress, low pH and starvation. Upon primary stress, E. coli develops increased resistance against a secondary unrelated stressor such as antibiotics.
Evaluating Double Agar Overlay Assay and Flow Cytometry as Methods for Characterizing Competition between T4 and T7 Bacteriophages in Escherichia coli C600
09/01/2016
Volume 20
Fall 2015 / Winter 2016
Bacteriophages are the most abundant viruses found on earth with population densities approximately ten fold of prokaryotes in the same environments. As a result, interspecies competition likely plays a large role in regulating phage populations in the environment.