Current JEMI Papers

Growth phase of host Escherichia coli MG1655 influences T4 and T7 bacteriophage replication patterns during coinfection.

09/09/2018

Naomi Fettig, Amanda Ryken, Nicole Mar, and Angela Sunario​

Volume 22
Fall 2017 / Winter 2018

SUMMARY T4 and T7 bacteriophages are proposed to compete during coinfection of Escherichia coli, impacting their replication relative to monoinfection conditions. During monoinfection of E.

Development of Tools for Genomic-Editing of the P1 Periplasmic Region of YidC: CRISPR/Cas9 Coupled with Lambda Red Recombineering in Escherichia coli MG1655.

09/09/2018

Alison Fong, Jenny He, Lucas Chang, Shawn Zhang​

Volume 22
Fall 2017 / Winter 2018

SUMMARY YidC is a conserved and essential protein that plays a role in the insertion and folding of Sec-dependent and Sec-independent membrane proteins. It has six transmembrane domains and one large 35 kDa periplasmic domain (P1) positioned between transmembrane domains 1 and 2.

Assessing the contributions of the multidrug efflux pump components acrE and acrA in mediating resistance to kanamycin in E. coli BW25113 : Steps towards the generation of acrA/acrE double mutants using CRISPR/Cas9 system.

09/09/2018

Valentin Gabeff, Camille Mitchell, Ivan Tai, Yun Chen Wu​

Volume 22
Fall 2017 / Winter 2018

SUMMARY Efflux pumps in Escherichia coli are responsible for their intrinsic resistance to antimicrobial compounds. AcrAB-TolC and AcrEF-TolC are structurally similar efflux pumps that have been proposed to be responsible for the export of kanamycin.

Exposure of Exponential Phase Escherichia coli to Ampicillin and Gentamicin Does Not Confer Cross-Protection Against T7 Bacteriophage-Induced Lysis

09/09/2018

Amanda Krystal, Reiko Okamoto, Andrea Sze, Zachary Weiss​

Volume 22
Fall 2017 / Winter 2018

SUMMARY Bacteria like Escherichia coli employ sensing and gene regulatory systems to respond to stress. The sigma factor RpoS has been shown to play a role in response to stressors like antibiotics.

O16 Antigen Confers Resistance to Bacteriophage T4 and T7 But Does Not Reduce T4/T7 Adsorption in Escherichia coli K-12.

09/09/2018

Steven Lee, Brett de Bie, Jessica Ngo, Jorden Lo​

Volume 22
Fall 2017 / Winter 2018

SUMMARY Expression of O16 antigen in MG1655 Escherichia coli K-12 was previously shown to be sufficient to confer resistance to T4 bacteriophage mediated cell-lysis. It was further demonstrated that O16 antigen confers resistance against T4 bacteriophage by preventing adsorption.

Sub-lethal treatment of Escherichia coli strain B23 with either β-lactam or Aminoglycoside Antibiotics May Delay T7 Bacteriophage-mediated Cell Lysis

09/09/2018

Haoran Li, Wumian Wang, Yiwei Yang, Myra Lam​

Volume 22
Fall 2017 / Winter 2018

SUMMARY With increasing rates of antibiotic resistance, there is renewed interest in the utilization of bacteriophages to treat pathogenic bacteria. However, the complex interplay between bacteria, phage, and antibiotic treatments is not well understood. A recent study by Hardman et al.

Plasmid mediated complementation of wza in Escherichia coli K30 strain CWG281 restores erythromycin sensitivity

09/09/2018

Amy Pochanart, Matt Richardson, Peter Truong, Jessica Wang

Volume 22
Fall 2017 / Winter 2018

SUMMARY In Escherichia coli serotype K30, the outer membrane channel protein Wza, involved in capsule secretion, has been genetically linked to macrolide sensitivity. Deletion of wza in erythromycin-sensitive E. coli K30 confers resistance to erythromycin.

Sub-Lethal Gentamicin Treatment of Escherichia coli UB1005 Induces the Release of Soluble Factors that Reduce Susceptibility to T7 Bacteriophage Infection

07/13/2017

Blair Hardman, David Jure Hunt, Danial Mojaab, Aaron Naor​

Volume 21
Fall 2016 / Winter 2017

Escherichia coli have evolved numerous mechanisms to resist infection by bacteriophages. Here, we investigate if resistance to phage T7 infection involves the gentamicin-induced release of soluble factors, such as lipopolysaccharide (LPS).

Sodium Dodecyl Sulfate-Eethylenediaminetetraacetic Acid Sensitive Phenotype Associated with ompC Deficient Escherichia coli Strains is Observed Primarily in Cells Growing in Stationary Phase and Less So in Cells Growing in Log Phase

07/13/2017

Rachel Sheinfeld, Marie-Soleil Smith, Stephanie Valdes​

Volume 21
Fall 2016 / Winter 2017

The highly conserved, multi-component Mla inter-membrane lipid trafficking system is proposed to be involved in maintaining outer membrane lipid asymmetry. It has been shown that MlaA interacts with both the OmpC and OmpF porins.

RcsB-Deficient Escherichia coli K-12 Do Not Exhibit Decreased Intrinsic Resistance Towards Antibiotics That Target the Cell Wall

07/13/2017

Paul McDade, Angela Wang, Vivian Wang, Clement Yau​

Volume 21
Fall 2016 / Winter 2017

Understanding intrinsic antibiotic resistance is crucial in face of the many challenges arising in therapeutic antibiotic use.

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