Current JEMI Papers

Deletion of Escherichia Coli K30 Type I Capsule Assembly Gene wzc Confers Resistance to The Antibiotic Erythromycin in Solid Media

07/13/2017

Aria Jazdarehee, Jennifer Joy Anderson, Devon Morrison, William Pardoe​

Volume 21
Fall 2016 / Winter 2017

Escherichia coli K30 produce a Type I (Colanic Acid) capsule which protects the bacterium from environmental stresses, including host immune responses. It is unknown how the presence of Type I capsule affects antibiotic resistance in the bacterium.

Ribosomal 16S rRNA Sequencing Shows That A Putative yidC Escherichia coli Knockout Strain Is Actually Rhizobium

07/13/2017

Ida Hooshmand, Manish Hosanee, Jewel Ocampo, Wendy Yu​

Volume 21
Fall 2016 / Winter 2017

To study the function of the major periplasmic domain of the inner membrane insertase YidC, Choi et al. (JEMI, Volume 20) constructed two chromosomal yidC knockout strains of Escherichia coli, WJKC-1 and WJKC-2.

Use of Matrix Assisted Laser Desorption/Ionization Time-of- Flight Mass Spectrometry (MALDI-TOF-MS) for Detection of Escherichia coli in Clinical Urine Samples

07/13/2017

Chantal Holm, Kingsley Donkor, Ken Wagner. Naowarat Cheeptham

Volume 21
Fall 2016 / Winter 2017

Urinary tract infections (UTIs) are very common and are becoming a concern in the medical community due to the growing prevalence of antimicrobial resistance in causative pathogens.

Role of the Rcs Phosphorelay in Intrinsic Resistance to Penicillin, Phosphomycin, and Cefsulodin in Escherichia coli K12

07/13/2017

James Wang, Michael Woo, Chris Yan

Volume 21
Fall 2016 / Winter 2017

The Regulator of Capsule Synthesis (Rcs) of Escherichia coli detects cell wall stress and elicits a stress response. The Rcs phosphorelay system consists of the outer membrane kinase sensor RcsF, the inner membrane kinases RcsC and RcsD, and then response regulator RcsB.

Investigation of Wza in Erythromycin Sensitivity of Escherichia coli K30 E69 by Genetic Complementation

07/13/2017

Brian Yuen, Jerome Ting, Kevin Kang, Timothy Wong​

Volume 21
Fall 2016 / Winter 2017

Escherichia coli K30 strains express group 1 capsule that is polymerized and transported by the Wzy-dependent pathway. This involves proteins encoded by genes wza, wzb, and wzc.

Reduced Bacteriophage T7 Infectivity of Gentamicin Treated Escherichia Coli UB1005 Is Likely Not a Result of Treatment- Induced Release of Cellular Factors Into The Culture Supernatant

07/13/2017

Manveer Nagra, Rachel Miller, Freddy Francis​

Volume 21
Fall 2016 / Winter 2017

Pre-treatment of Escherichia coli UB1005 with sub-lethal concentrations of gentamicin has been shown to reduce the infectivity of Bacteriophage T7. However, levels of infectivity has been shown to increase after a wash following antibiotic treatment.

Development of a Microtiter Plate Assay for Real Time Analysis of T7 Bacteriophage Mediated Lysis of Escherichia coli

07/13/2017

David Cho, Irene Lau, Michael Li, Dave Zhu​

Volume 21
Fall 2016 / Winter 2017

When stressed, Escherichia coli releases soluble factors such as outer membrane vesicles. The release of these factors in the extracellular milieu has been shown to prevent bacteriophage infection in some cases.

Development of a Plasmid-Based System for Studying DNA Repair Mechanisms in Escherichia coli

07/13/2017

Angela Chang, Vivian Chau, Julius Landas, Yvonne Pang​

Volume 21
Fall 2016 / Winter 2017

Nucleotide excision repair is a DNA repair mechanism involved in the repair of ultraviolet radiation damage. The key proteins in this system are the four Uvr proteins: UvrA, UvrB, UvrC, and UvrD.

The Rcs-Phosphorelay Pathway Is Not Essential for Intrinsic Antibiotic Resistance to β-lactam Antibiotics in Escherichia coli

07/13/2017

Daniel Chen, Alex Yoon, Janice Lee, Julianna Cheung​

Volume 21
Fall 2016 / Winter 2017

Rcs phosphorelay pathway in Escherichia coli is a response system that senses outer membrane stress conditions and regulating capsule and biofilm formation which can result in antibiotic resistance.

Soluble LPS May be a More Potent Competitive Inhibitor to Polymyxin B Than Escherichia coli O9a:K30 Capsular Polysaccharides

07/13/2017

Aska Fang, Frank Liu, Tiffiny Zang, Steven Zhao​

Volume 21
Fall 2016 / Winter 2017

Escherichia coli expresses an extracellular layer of capsular polysaccharide (CPS) that confers protection from environmental stressors such as desiccation and antibiotics.

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