Current JEMI Papers

otsA single gene knock-out in Escherichia coli increases cell sensitivity to outer membrane destabilization by SDS and EDTA treatment

09/26/2019

Albert Chang, Yu Gu, Kenny Hui, Chris Xi

Volume 24
Fall 2018 / Winter 2019

Trehalose is a disaccharide consisting of two glucose molecules and is produced by various organisms, including Escherichia coli via the otsBA operon. It is

Knockout of OmpF and OmpC does not affect kanamycin susceptibility in two different strains of Escherichia coli K-12

09/26/2019

Aidan Canil, Navid Saleh, Jerry Chan

Volume 24
Fall 2018 / Winter 2019

OmpF and OmpC are key outer membrane diffusion porins in Escherichia coli. Previous studies have tested OmpF and OmpC mutants for resistance to the antibiotic kanamycin, with conflicting results. We set out to determine if the inconsistent results were due to the different E.

Pre-treatment with cell-wall targeting antibiotics and aminoglycosides does not confer cross protection to cell-wall targeting antibiotics in Escherichia coli despite upregulation of rprA

09/26/2019

Josh Bowman, Robin Couput, Anthony Pookpun, Kendrew Wong

Volume 24
Fall 2018 / Winter 2019

The Rcs phosphorelay system is a signaling pathway in Escherichia coli involved in cell envelope stress response. RcsB, located in the cytoplasm, activates

Negative Stain Electron Microscopic Analysis Suggests O Antigen Expression in Escherichia coli strain K-12 May Prevent T4 Interactions with the Bacterial Surface

09/26/2019

Naser (Milad) Biparva, Arya Ghazizadeh, Thomas Hoang, Sunny Sun

Volume 24
Fall 2018 / Winter 2019

Expression of O16 antigen on the surface of Escherichia coli K-12 has been shown to confer resistance to bacteriophage T4-mediated lysis, however, the underlying mechanism of resistance is poorly understood.

Bacterial Genome Editing Using CRISPR/Cas9 Coupled with Lambda Red Recombineering for Potential Generation of an Antigen 43 Knockout

09/09/2018

Daniel Chan, Felix Ma, Abigail Moraes, Ian Lee​

Volume 22
Fall 2017 / Winter 2018

SUMMARY To study the effect of differential Antigen 43 expression on biofilm formation, we attempted to generate a knockout mutant of the flu gene in a well-characterized Escherichia coli lab strain, MG1655.

Sublethal Concentrations of Antibiotics Do Not Markedly Delay T7 Bacteriophage-Mediated Lysis Between Wild-Type and rpoS, and oxyR Mutants of Escherichia coli

09/09/2018

Mahmoud Alnasleh, Habibur Rahman, Syed Shuja Raza, Boyuan Zheng​

Volume 22
Fall 2017 / Winter 2018

SUMMARY T7 bacteriophages are obligatory lytic phages that infect bacterial cells, and hijack host cell machinery to replicate their genome. Bacteriophage infection acts as an environmental stressor for E. coli, like other known factors such as antibiotics, and nutrient deprivation.

Treatment of Escherichia coli K-12 with sub-inhibitory concentrations of antimicrobial agents does not induce RpoSmediated cross-protection to T7 bacteriophage infection

09/09/2018

Polina Beskrovnaya, Nicole Janusz, Lana Omazic, Frances Perry

Volume 22
Fall 2017 / Winter 2018

SUMMARY The Rcs-phosphorelay pathway is used by Escherichia coli to express the alternative sigma factor RpoS in response to extracellular stressors. It has been proposed that RpoS may mediate cross-protection from both antibiotics and bacteriophage infection.

Investigating the Effects of Bis (2-ethylhexyl) Phthalate (DEHP) on the Growth of Escherichia coli strain BL21

09/09/2018

Jordan Brundrett, Juma Orach, Lukas Sonderegger, Tony Yan​

Volume 22
Fall 2017 / Winter 2018

SUMMARY Phthalates (also known as phthalate esters) are organic chemicals used as plasticizers in a wide range of food packaging, containers, and other household plastic objects. Humans are exposed to phthalates through the air and by ingestion.

Differences in growth and antibiotic susceptibility in Escherichia coli stringent response mutants are conferred in minimal nutritional conditions irrespective of amino acid limitation.

09/09/2018

Massimo Cau, Aidan Goodall, Leo Liu, Nicole Liu​

Volume 22
Fall 2017 / Winter 2018

SUMMARY The rise of antibiotic resistance crisis is a critical challenge facing healthcare providers around the world. To combat this phenomenon, we must understand underlying cellular processes that contribute to antibiotic resistance, such as the stringent response.

Genetic characterization and investigation of kanamycin susceptibility of ompC and ompF single gene deletion mutants of Escherichia coli K-12

09/09/2018

Chuan Chuan Chang, Ching Wing Yan, Regina Onal, and Yue Zhang​

Volume 22
Fall 2017 / Winter 2018

SUMMARY OmpF and OmpC are proteins that form porins in the outer membranes of Gram-negative bacteria.

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